Intratumor injection of recombinant attenuated salmonella carrying Mycobacterium tuberculosis heat shock protein 70 and herpes simplex virus thymidine kinase genes to suppress murine melanoma growth.
- Author:
Shuguang ZENG
1
;
Qicai LIU
;
Suwen WANG
;
Ximao PENG
;
Jincai ZHANG
;
Jiren ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bacterial Proteins; genetics; immunology; Cancer Vaccines; genetics; immunology; pharmacology; Genetic Therapy; methods; HSP70 Heat-Shock Proteins; genetics; immunology; Melanoma, Experimental; microbiology; pathology; therapy; Mice; Mice, Inbred C57BL; Mycobacterium tuberculosis; genetics; Salmonella typhimurium; genetics; immunology; Simplexvirus; enzymology; genetics; Skin Neoplasms; therapy; Thymidine Kinase; genetics; immunology; Vaccines, Attenuated; genetics; immunology; pharmacology; Vaccines, DNA; genetics; immunology; pharmacology
- From: Journal of Southern Medical University 2012;32(1):101-105
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effection of suppression murine melanoma growth by Intratumor injection of recombinant attenuated salmonella carrying heat shock protein 70 and herpes simplex virus thymidine kinase genes.
METHODSPlasmids PCMV-mtHSP70-IRES-TK were electro-transferred into salmonella typhimurium SL7207 to construct recombinant salmonella typhimurium. In vivo, Recombinant bacteria were injected into the mouse melanoma and the antitumor effection was observed. The survival period was recorded and safety analysis for this vaccine in each group.
RESULTSIn vivo, the mtHSP70/HSV-tk recombinant bacteria can suppress tumor growth significantly and extend survival. After recombinant Salmonella, 10(9) CFU/mL, was administered as an intratumoral injection, No diarrhea were observed. During therapy, body weight did not change markedly.
CONCLUSIONResults of the animal experiment suggests intratumor injection of recombinant attenuated salmonella typhimurium containing mtHSP70 and HSV-tk genes, has targeting ability against B16 tumor cell and could significantly inhibit tumor growth .
