Effects of Bushen Huoxue Fang on rat cardiac fibroblast proliferation and collagen production in vitro.
- Author:
Xinying MA
1
;
Minghui YANG
;
Jinsong CHEN
;
Yi LIU
;
Congze PAN
;
Jinzhuo FAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cell Proliferation; drug effects; Cells, Cultured; Collagen Type I; biosynthesis; Collagen Type III; biosynthesis; Drugs, Chinese Herbal; pharmacology; Fibroblasts; cytology; metabolism; Fibrosis; prevention & control; Myocardium; cytology; metabolism; pathology; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2012;32(1):122-124
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Bushen Huoxue Fang on the proliferation of rat cardiac fibroblasts and collagen production in the cells.
METHODSRat cardiac fibroblasts were isolated and cultured in DMEM containing 10% (group A) or 20% (group B) or no (group C) serum from rats treated with Bushen Huoxue Fang, with cells cultured in DMEM containing 10% FBS as the control (group D). After 72 h of cell culture, the proliferation of the fibroblasts was detected using CCK-8 kit, and collagen mRNA and protein expressions were examined using RT-PCR and Western blotting, respectively.
RESULTSCompared with that in groups C and D, the cell proliferation decreased significantly in groups A and B, and especially in the latter (P<0.05). RT-PCR demonstrated significant reductions of the mRNAs of type 1 and 3 collagens in groups A and B (P<0.05), and their protein levels were also significantly lowered (P<0.05).
CONCLUSIONBushen Huoxue Fang can effectively inhibit the proliferation of rat cardiac fibroblasts and reduced collagen type 1 and 3 productions in the cells in vitro.
