Determination of melamine by high-performance liquid chromatography-mass spectrometry in urinary calculi.
- Author:
Wenqi WU
1
;
Luping WANG
;
Lili OU
;
Yeping LIANG
;
Zanlin MAI
;
Shujue LI
;
Chichang SHAN
;
Jian YUAN
;
Guohua ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Dairy Products; adverse effects; Female; Food Contamination; Humans; Infant; Male; Mass Spectrometry; methods; Triazines; analysis; Urinary Calculi; chemically induced; chemistry
- From: Journal of Southern Medical University 2012;32(1):125-127
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a high-performance liquid chromatography-mass spectrometry (LC/MS)-based method for efficient determination of melamine in urinary calculi in children taking melamine-contaminated formula milk powder.
METHODSLC/MS was employed to determine the contents of melamine in urinary calculi surgically removed from 17 children with a history of taking melamine-contaminated milk powder and in 4 samples of uric acid stone from adults. The positive ionization mode of electro-spray ionization source was used, and the limit of melamine determination was 0.1 mg/kg.
RESULTSNo melamine was detected in the 4 uric acid stone samples from adults. Melamine was detected in 4 samples of urinary calculi from the 17 children, with the concentration ranging from 0.8 to 64 mg/lkg.
CONCLUSIONLC/MS is simple and effective for detecting melamine in urinary calculi, which is helpful to the treatment and follow-up.
