Effect of sodium butyrate on apoptosis and stromal interaction molecule and Orai1 activity in human colon cancer HCT-116 cells in vitro.
- Author:
Suxia SUN
1
;
Wenjun LI
;
Siqiang CHEN
;
He ZHANG
;
Shaozhen YU
;
Minhong ZHANG
;
Fei ZOU
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Butyrates; pharmacology; Calcium Channels; metabolism; HCT116 Cells; Histone Deacetylase Inhibitors; pharmacology; Humans; Membrane Proteins; metabolism; Neoplasm Proteins; metabolism; ORAI1 Protein; Stromal Interaction Molecule 1
- From: Journal of Southern Medical University 2012;32(2):189-192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the mechanism underlying sodium butyrate (NaB)-induced apoptosis of a human colon cancer cell line HCT-116.
METHODSThe apoptosis of HCT-116 cells induced by NaB was confirmed by hoechst33342 staining and AnnexinV+ PI assay. The changes in the intracellular localization of stromal interaction molecule (STIM1) and Orai1 following NaB treatment were detected by immunofluorescence technique. Western blotting was used to investigate the protein expression levels of STIM1 and Orai1.
RESULTSNaB induced apoptosis and caused translocation and colocalization of STIM1 and Orai1 in HCT-116 cells.
CONCLUSIONThe apoptosis of human colon cancer cells induced by NaB is correlated to the redistribution of STIM1 and Orai1.
