The study of acoustically evoked short latency negative responses in normal guinea pigs
10.3760/cma.j.issn.1673-0860.2015.12.010
- VernacularTitle:正常听力豚鼠声诱发短潜伏期负电位的研究
- Author:
Shaohua ZHANG
1
;
Jin CHEN
;
Chenxi LIN
;
Dongxiao NONG
;
Anzhou TANG
Author Information
1. 民航总医院耳鼻咽喉头颈外科
- Keywords:
Evoked potentials,auditory,brain stem;
Vestibular nucleus;
Perceptual masking;
Guinea pigs
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2015;50(12):1015-1019
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare acoustically evoked short latency negative responses (ASNR) elicited from normal and profound hearing loss guinea pig ears and to confirm their vestibular nuclei origination.Methods Forty healthy guinea pigs were employed in the experiment, which were randomly divided into the control group (8 subjects, 16 ears), the masking group (16 subjects, 32 ears) and the deafened group (16 subjects, 32 ears).Air conductive white noise was chosen for masking.Masking dilemma was avoidable by an appropriate 15 dB gap between stimulus and masking sound.Both the nasking group and the deafened group were further divided into ASNR group and non-ASNR group based on the presence of ASNR.Electrolytic lesion was conducted to the vestibular nuclei, followed by ABR/ASNR recording.The lesioned brainstem slices were microscopically verified.Results In the masking group, ASNR were present in 24 ears (75.0％ , 24/32) and 12 ears (46.2％ , 12/26) in deafened group, showing statistically higher presence rate for masking group (x2 =5.07, P =0.024).There were no significant differences for the ASNR threshold and latency between the masking ASNR group and the deafened ASNR group.For the two ASNR groups, electrolytic destruction to the vestibular nuclei subsequently eliminated the ASNR.Brainstem slice proved the accurate sites of electrolytic lesion.Conclusion Moderate white noise masking prevents hearing system potential overlap without affecting vestibular system, therefore, ASNR is successfully elicited in normal guinea pigs.Both ASNRs from normal and deafened guinea pigs are of similar natures and origination from, the vestibular nuclei.
