Proliferation effect of neural stem cell of ginsenoside Rg1 in vitro.
- Author:
Pengwei ZHUANG
1
;
Yanjun ZHANG
;
Tan PANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Ginsenosides; pharmacology; Multipotent Stem Cells; drug effects; physiology; Neurons; drug effects; physiology; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction
- From: China Journal of Chinese Materia Medica 2009;34(4):443-446
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the proliferation effect of neural stem cells (NSCs) of ginsenoside Rg1 in vitro.
METHODNSCs from the embryonic rat (E14) were isolated, 10 mg x L(-1) BrdU were added in the medium. The NSCs were incubated together with 1, 10 micromol x L(-1) ginsenoside Rg1 for 48 hours, control group were setup. Then BrdU positive cell number were detected and counted by fluorescence microscop. The Hes1 and Mash1 mRNA expression were detected by real-time RT-PCR.
RESULTThe number of BrdU positive cells in 10 micromol x L(-1) ginsenoside Rg1 group was increased significantly compared with the control group (40.5 +/- 10.9 vs 26.2 +/- 6.0, P < 0.01), and the Hes1 mRNA expression were increased significantly (1.00 +/- 0.11) vs (1.28 +/- 0.14), P < 0.05.
CONCLUSIONginsenoside Rg1 can promote the proliferation of neural stem cells in vitro, and this effect may be induced by the up-regulation Hes1 expression.
