Extraction of lobetyolin from codonopsis with supercritical CO2.

Author: Tongju LIU ¹ ; Shufen LI ; Jiang MIN ; Xiaomei BAO
Author Information

1. Key Laboratory for Green Chemical Technology of State Education Ministry, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Publication Type:Journal Article
MeSH: Carbon Dioxide; chemistry; Chemical Fractionation; methods; Codonopsis; chemistry; Drugs, Chinese Herbal; chemistry; Ethanol; chemistry; Polyacetylenes; chemistry
From: China Journal of Chinese Materia Medica 2009;34(5):560-563
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo develop a green and rapid method for extraction of lobetyolin from C. pilosula.
METHODExtraction of lobetyolin from C. pilosula with supercritical carbon dioxide in the presence of ethanol was studied. The effects of pressure, temperature, volume of cosolvent and extraction time on efficiency and their interactive relationships were discussed, based on central composite design and response surface methodology (RSM).
RESULTThe key effect factor was volume of cosolvent. The extraction yield of lobetyolin was 0.078 6 mg x g(-1) when C. pilosula (40-60 mesh) was extracted at 30 MPa, 60 degrees C and 2 L x min(-1) (as CO2 in normal pressure and temperature) for 100 minutes with supercritical CO2 and 1 mL x min(-1) ethanol as dynamic cosolvent.
CONCLUSIONThis result is better than that obtained from traditional method. Therefore, the optimized process is valuable for extraction of lobetyolin from C. pilosula.