Dracorhodin perchlorate inhibit high glucose-induced connective tissue growth factor formation in human mesangial cells.
- Author:
Yonghui WANG
1
;
Quansheng WANG
;
Jianguo LIU
;
Lixiao ZHANG
;
Bing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Benzopyrans; pharmacology; Connective Tissue Growth Factor; genetics; metabolism; Dose-Response Relationship, Drug; Gene Expression Regulation; drug effects; Glucose; pharmacology; Humans; Mesangial Cells; drug effects; metabolism; RNA, Messenger; genetics; metabolism; Time Factors
- From: China Journal of Chinese Materia Medica 2009;34(7):896-899
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of dracorhodin perchlorate on high glucose-induced connective tissue growth factor (CTGF) expression in human mesangial cells (HMC), and its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN).
METHODThe HMC were divided into low glucose group (LG group, 5.5 mmol x L(-1) D-glucose), high glucose group (HG Group 25 mmol x L(-1) D-glucose), each group was located with dracorhodin perchlorate (7. 5 micromol x L(-1)) as comparison, and was examined at 24, 48 h. The expression of CTGF mRNA was assessed by semi quantatiue RT-PCR, and the expression of CTGF protein was assessed by Western blot.
RESULTCompared to HG group, the expression of CTGF mRNA and protein were reduced in LG group after stimulating 24, 48 hours (P < 0.01). Compared to HG group, the expression of CTGF mRNA and protein were reduced in HG group by added with dracorhodin perchlorate after stimulating 24, 48 hours (P < 0.01).
CONCLUSIONDracorhodin perchlorate can inhibit high glucose-induced connective tissue growth factor expression in human mesangial cells, and this may be its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN).
