Preliminary study on human embryonic kidney cell line HEK-293 after porcine endogenous retrovirus infection.
- Author:
Ping YU
1
;
Li ZHANG
;
Hong BU
;
Sheng-fu LI
;
You-ping LI
;
Jing-qiu CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; DNA, Viral; analysis; Embryo, Mammalian; Endogenous Retroviruses; isolation & purification; pathogenicity; Gene Amplification; Gene Products, gag; biosynthesis; genetics; Genes, gag; Humans; Kidney; metabolism; virology; RNA, Messenger; biosynthesis; genetics; Swine
- From: Chinese Journal of Pathology 2005;34(4):220-223
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the infectivity of porcine endogenous retrovirus (PERV) via in vitro infection of human embryonic kidney cell line HEK-293.
METHODSPERV particles were detected by immunoelectron microscopy. PERV DNA and mRNA were studied in HEK-293 24 hours after the infection using polymerase chain reaction and reverse transcriptase-PCR respectively. The PERV types were also analyzed. PERV-gag protein was observed by confocal microscopy.
RESULTSRetroviral particles were round under electron microscope. PERV-gag pol gene and gag protein were detected and expressed in the infected HEK-293 cells. The types of PERV were PERV-A and PERV-B. PERV-gag protein was also identified in the cytoplasm of infected cells by confocal microscopy.
CONCLUSIONSPERV is able to infect HEK-293 cell line in vitro; types of PERV-gag protein is also expressed as a result. Further studies are thus necessary in order to evaluate the possibility of xenozoonoses in pig-to-human xenotransplantation.
