Effect of wortmannin on endothelial cell proliferation and migration induced by high glucose Müller cell conditioned medium.
- Author:
Yue-hui WANG
1
;
Xi-yuan WANG
;
Xin-rui WANG
;
Jin-song NI
;
Ying XIN
;
Bo SHI
;
Jia-xiang WU
Author Information
- Publication Type:Journal Article
- MeSH: Androstadienes; pharmacology; Animals; Cell Cycle; drug effects; Cell Movement; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Culture Media, Conditioned; pharmacology; Endothelial Cells; cytology; Female; Glucose; Humans; Male; Neovascularization, Pathologic; Phosphatidylinositol 3-Kinases; antagonists & inhibitors; Rabbits; Retina; cytology
- From: Chinese Journal of Pathology 2005;34(4):229-232
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of wortmannin on endothelial cells proliferation and migration induced by high glucose Müller cell conditioned medium (HGMCM).
METHODSImmunofluorescence, flow cytometry and Boyden chamber migration models were used to analyze the effect of wortmannin on endothelial cells.
RESULTS50 nmol/L wortmannin could significantly inhibit the proliferation and migration of endothelial cells induced by HGMCM. The percentage of endothelial cells in S phase was obviously decreased [from (37.82 +/- 0.57)% to (21.91 +/- 0.23)%, P < 0.01], while there was an increase in the percentage of cells in G(0)/G(1) phase [from (54.57 +/- 1.19)% to (65.59 +/- 0.41)%, < 0.01] and G(2)/M phase (< 0.05).
CONCLUSIONWortmannin can inhibit proliferation and migration of endothelial cells induced by HGMCM, suggesting that wortmannin carries an anti-angiogenetic ability in diseased retina.
