Sudy on the activation of early growth response factor-1 by silica dioxide and its signal pathway.
- Author:
Ling CHU
1
;
Zhong-yuan JIN
;
Hai-ying JIANG
;
Yong-bin HU
;
Qing-fu ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Butadienes; pharmacology; Cells, Cultured; Early Growth Response Protein 1; biosynthesis; genetics; physiology; Enzyme Inhibitors; pharmacology; Gene Expression Regulation; Macrophages; metabolism; Mice; Mitogen-Activated Protein Kinase 1; metabolism; Mitogen-Activated Protein Kinase 3; metabolism; Nitriles; pharmacology; RNA, Messenger; biosynthesis; genetics; Signal Transduction; Silicon Dioxide; pharmacology; p38 Mitogen-Activated Protein Kinases; metabolism
- From: Chinese Journal of Pathology 2005;34(5):293-296
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo discuss the role of early growth response factor (Egr)-1 and it's upstream signaling pathway in the development of silicosis.
METHODSThe expression and localization of Egr-1 were analyzed by immunofluorescence and in-situ hybridization. The activity of Egr-1 was observed in treated cells by using a reporter plasmid and EMSA, the activity of ERK1/2 in RAW264.7 incubated with SiO(2) by using a kinase assay, and further by using a kinase inhibitor assay to investigate the role of upstream kinase in the signal pathway of the activation of Egr-1.
RESULTSThe obvious increase of expression and transcription of Egr-1 was observed shortly after being treated by silica and its activity increased abruptly. There was an increase of the activity of ERK1/2 in RAW264.7 cells treated, which reached a peak at 30 minutes. The expression and transcription of Egr-1 decreased maniferstly after using kinase inhibitors.
CONCLUSIONEgr-1 expression can be induced by silica dioxide in RAW264.7 cells, and the ERK1/2, p38 kinases may take part in this process which suggest the pathway of SiO(2), ERK1/2, p38 and Egr-1 may play an important role in the development of silicosis.
