Analysis of bcl-6 protein expression and gene rearrangement in diffuse large B-cell lymphoma.
- Author:
Da-liu MIN
1
;
Hai-long XIA
;
Xiao-yan ZHOU
;
Meng-hong SUN
;
Wen-tao YANG
;
Tai-ming ZHANG
;
Ai-hua ZHENG
;
Da-ren SHI
Author Information
- Publication Type:Journal Article
- MeSH: Diagnosis, Differential; Female; Gene Rearrangement; Humans; In Situ Hybridization, Fluorescence; Lymphoma, B-Cell; genetics; metabolism; pathology; Lymphoma, Large B-Cell, Diffuse; genetics; metabolism; pathology; Male; Middle Aged; Neoplasm Staging; Neprilysin; metabolism; Proto-Oncogene Proteins c-bcl-6; genetics; metabolism; Pseudolymphoma; genetics
- From: Chinese Journal of Pathology 2005;34(6):327-331
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate bcl-6 protein expression and gene rearrangement patterns in diffuse large B-cell lymphoma (DLBCL) and their clinicopathologic significance.
METHODSImmunohistochemical studies for bcl-6 and CD10 proteins were performed on 51 cases of DLBCL paraffin-embedded tissues (including 22 nodal samples and 29 extranodal samples) and 10 cases of reactive lymphoid hyperplasia (RLH) paraffin-embedded tissues. Interphase fluorescence in-situ hybridization (FISH) with dual color breakapart probe was also used to identify rearrangement of bcl-6 gene in 32 cases of nodal DLBCL tissues (including 22 paraffin-embedded samples and 10 fresh samples) and 5 cases of RLH paraffin-embedded tissues.
RESULTS(1) The rates of bcl-6 protein expression in nodal DLBCL, extranodal DLBCL and RLH were 72.7% (16/22), 75.9% (22/29) and 100.0% (10/10) respectively. The rates of CD10 expression were 40.9% (9/22), 41.4% (12/29) and 100.0% (10/10) respectively. All lymphoma samples which expressed CD10 also showed co-expression of bcl-6 protein. (2) The co-expression of bcl-6 and CD10 was observed in 40.9% (9/22) nodal DLBCL and 41.4% (12/29) extranodal DLBCL. Low clinical stage (stage I and II) was more frequently observed in cases with co-expression of bcl-6 and CD10 (P < 0.05). (3) The rates of bcl-6 gene rearrangement in nodal DLBCL was 28.1% (9/32), with 27.3% (6/22) in paraffin-embedded tissues and 30.0% (3/10) in fresh tissues. There was no statistically significant difference found between the two groups (P > 0.05). Bcl-6 gene rearrangement was not found in all the 5 cases of RLH, and there was a significant difference between RLH and DLBCL (P < 0.05).
CONCLUSIONSThe rate of bcl-6 protein expression is high in DLBCL cases, and the detection of bcl-6 and CD10 protein co-expression may help in the diagnosis and differential diagnosis of DLBCL. Those DLBCL cases with co-expression of bcl-6 and CD10 may also have a better prognostic implication. On the other hand, bcl-6 gene rearrangement can be identified by interphase FISH with dual color breakapart probe in both paraffin-embedded and fresh lymphoma tissues.
