Preliminary analysis of mutations in exon 5 of IkappaBalpha gene in Hodgkin lymphoma.
- Author:
Xiao-jian LIU
1
;
Wen-tao YANG
;
Xiao-yan ZHOU
;
Qing YAN
;
Da-ren SHI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Base Sequence; DNA Mutational Analysis; DNA, Neoplasm; genetics; Exons; genetics; Female; Gene Deletion; Hodgkin Disease; genetics; Humans; I-kappa B Proteins; genetics; Male; Middle Aged; Molecular Sequence Data; NF-KappaB Inhibitor alpha; Point Mutation
- From: Chinese Journal of Pathology 2005;34(6):341-344
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the mutations in exon 5 of IkappaBalpha gene and their significance in Hodgkin lymphoma.
METHODSCD30-positive Reed-Sternberg cells and surrounding reactive lymphocytes were selected by laser microdissection technology from 6 microm-thick histologic sections of 7 Hodgkin lymphoma cells studied. Genomic DNA of these cells was extracted according to Qiagen micro DNAkit guide. Semi-nested polymerase chain reaction (PCR) and direct DNA sequencing were used to analyze exon 5 of IkappaBalpha gene.
RESULTSA mutation in exon 5 was identified in 3 of the 7 Hodgkin lymphoma cases. No mutation found in the surrounding reactive lymphocytes. The mutation site was located at codon 2084 (T-A, NT_026437), resulting in formation of a stop codon.
CONCLUSIONThe mutation identified in exon 5 of the IkappaBalpha gene in Hodgkin lymphoma may play a role in the pathogenesis of Hodgkin lymphoma.
