Immunohistochemical method for the detecting expression of transforming growth factor-beta1 in lung tissues of silica-treated mice.
- Author:
Wen-jie JI
1
;
Lei YANG
;
Jia-shun DING
;
Zheng-lun WANG
;
Cang LIU
;
Han-zhen HE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Immunohistochemistry; methods; Lung; chemistry; drug effects; pathology; Male; Mice; Pulmonary Fibrosis; etiology; pathology; Silicon Dioxide; pharmacology; toxicity; Silicosis; etiology; pathology; Time Factors; Transforming Growth Factor beta; analysis
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(3):182-184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protein expression of transforming growth factor-beta(1) (TGF-beta(1)) in lung tissues of silica-treated mice.
METHODSThe experimental mice were divided into control and silica groups. 0.2 g/kg body weight of silica was injected intratracheally in mice of silica group. Samples of lung tissue were collected 1, 3, 5, 7, 14 and 28 d after injection. The immunohistochemical method was used to analyze the protein expression of TGF-beta(1).
RESULTSIn control mice, the expression of TGF-beta(1) in lung tissue was slightly positive while it was markedly increased in silica-treated mice. The expression was significantly elevated from the 7th day to 14th day. The expression in alveolar macrophages reached the peak on the 5th day [(93.4% +/- 2.8%) vs (42.2% +/- 12.0%), P < 0.01].
CONCLUSIONTGF-beta(1) may play an important role in early development of silicosis.
