RT-PCR method for detecting the expression of transforming growth factor-beta1 gene in lung tissues of silica-treated mice.
- Author:
Wen-jie JI
1
;
Lei YANG
;
Zheng-lun WANG
;
Jia-shun DING
;
Cang LIU
;
Han-zhen HE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Gene Expression Regulation; drug effects; Lung; drug effects; metabolism; pathology; Male; Mice; Pulmonary Fibrosis; etiology; pathology; Reverse Transcriptase Polymerase Chain Reaction; methods; Silicon Dioxide; pharmacology; toxicity; Time Factors; Transforming Growth Factor beta; genetics
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(3):185-187
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the gene expression of transforming growth factor-beta(1) (TGF-beta(1)) in lung tissues of silica-treated mice.
METHODSThe experimental mice were divided into control and silica group. 0.2 g/kg body weight of silica was injected intratracheally in silica group. Samples of lung tissue were collected 1, 3, 5, 7, 14 and 28 d after injection. RT-PCR method was used to analyze the gene expression of TGF-beta(1) in lung tissue of silica-treated mice.
RESULTSThe expression of TGF-beta(1) gene in lung tissue elevated from the 3rd day (1.20 +/- 0.15) and the peak value was on the 7th day (1.74 +/- 0.19). Then the expression decreased from the 14th to 28th day. But there was still higher than control until the 28th day.
CONCLUSIONTGF-beta(1) may play an important role in silica-induced pulmonary fibrosis.
