Effects of organophosphates on cell proliferation and 45Ca uptake of neuroblastoma SH-SY5Y cells.
- Author:
Ming LI
1
;
Ping-an CHANG
;
Yu-xia LI
;
Wei LI
;
Yi-jun WU
Author Information
- Publication Type:Journal Article
- MeSH: Calcium Radioisotopes; metabolism; pharmacokinetics; Cell Division; drug effects; Cell Line, Tumor; drug effects; Humans; Insecticides; administration & dosage; Neuroblastoma; metabolism; pathology; Organophosphorus Compounds
- From:Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(3):200-202
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanism of cytotoxic effects of the organophosphates (OPs) with delayed neurotoxicity on human neuroblastoma cells.
METHODSThe proliferation of neuroblastoma SH-SY5Y cells was determined by MTT spectrometry. (45)Ca uptake was determined by adding (45)CaCl(2) and tri-o-cresyl phosphate (TOCP) or methamidophos into the cultured medium for the SH-SY5Y cells. The cells were incubated and then lysed and finally counted in a Beckman LS 6000 liquid scintillation spectrometer.
RESULTSMethamidophos stimulated the cell proliferation of SH-SY5Y at its lower concentrations (7 x 10(-7) mol/L to 7 x 10(-6) mol/L), with an increase by 28% at 7 x 10(-7) mol/L; however, it inhibited the proliferation at higher ones (7 x 10(-4) mol/L to 7 x 10(-3) mol/L) with 62% inhibition at 7 x 10(-3) mol/L. TOCP only inhibited the cell proliferation at high concentration (with 34% inhibition at 7 x 10(-3) mol/L) and markedly inhibited calcium uptake of the cells up to 55% at higher concentrations (1 x 10(-6) mol/L to 1 x 10(-4) mol/L); while the uptake was stimulated by OPs up to 241% of increase at lower concentrations (1 x 10(-9) mol/L to 1 x 10(-7) mol/L).
CONCLUSIONThe interference of growth in nerve cells and disturbance of calcium homeostasis may be involved in the mechanisms of neurotoxicity of OPs.
