The mechanism of JWA gene involved in oxidative stress of cells.
- Author:
Nan-ping WANG
1
;
Jian-wei ZHOU
;
Ai-ping LI
;
Hai-xia CAO
;
Xin-ru WANG
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Northern; Cell Line; Cell Line, Tumor; Gene Expression Regulation; drug effects; Glutathione; metabolism; HSP70 Heat-Shock Proteins; metabolism; HSP90 Heat-Shock Proteins; metabolism; Heat-Shock Proteins; genetics; metabolism; Humans; Hydrogen Peroxide; administration & dosage; Intracellular Signaling Peptides and Proteins; Malondialdehyde; metabolism; Oxidative Stress; RNA, Messenger; drug effects; genetics; metabolism
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(3):212-215
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the mechanism of gene JWA involved in oxidative stress under hydrogen peroxide (H(2)O(2)) exposure.
METHODSBoth MCF-7 (human breast cancer cell line) and WI-38 (human embryo lung fibroblast cell line) cells were treated with 1 mmol/L of H(2)O(2) with or without pre-incubation of taurine (tau). Malondialdehyde (MDA) and glutathione (GSH) contents in supernatant of cell culture were measured; RT-PCR and Western blotting were carried out for evaluation of the expressions of JWA mRNA and protein respectively. Heat shock proteins (HSP27, HSP70 and HSP90) were also analyzed.
RESULTSThe contents of MDA before and after H(2)O(2) treatment in MCF-7 cells were (0.531 +/- 0.038), (0.674 +/- 0.410) mmol/L respectively, (P < 0.01), while those in WI-38 cells were (0.572 +/- 0.035), (0.683 +/- 0.028) mmol/L respectively, (P < 0.01). The contents of GSH before and after H(2)O(2) treatment in MCF-7 cells were (0.053 +/- 0.002), (0.044 +/- 0.002) g/L respectively, (P < 0.01), while those in WI-38 cells were (0.058 +/- 0.002), (0.050 +/- 0.002) g/L respectively, (P < 0.01). The expression of JWA mRNA was down regulated, at 6 h it decreased by 68.4%, while in WI-38 cells no obvious change found. JWA protein and HSP27 showed markedly increased after H(2)O(2) treatment in both cells but not in similar extent.
CONCLUSIONOxidative stress signal pathways of JWA gene varied between cancer and non-cancer cell lines; JWA protein may have a similar function as HSP27 and act as an important signal molecule in H(2)O(2) induced cell injury.
