Studies on fluoride concentration and cholinesterase activity in rat hippocampus.
- Author:
Jin-xia ZHAI
1
;
Zhi-yong GUO
;
Chuan-lai HU
;
Qi-nan WANG
;
Qi-xing ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Acetylcholinesterase; metabolism; Animals; Blood-Brain Barrier; Butyrylcholinesterase; metabolism; Fluoride Poisoning; metabolism; Fluorides; pharmacokinetics; Hippocampus; metabolism; Male; Organ Size; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(2):102-104
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the accumulation of fluoride in rat hippocampus and its effect on cholinesterase activity.
METHODSRats were subchronically exposed to NaF, and fluoride concentration and cholinesterase activity in rat hippocampus were determined.
RESULTSFluoride concentration in rat hippocampus was significantly correlated with the dosage of fluoride, and there were significant differences among high dosage group [(13.03 +/- 1.79) micro g/g], low dosage group [(9.83 +/- 0.92) micro g/g] and control [(8.27 +/- 1.11) micro g/g], P < 0.01. Acetylcholinesterase activities among three groups [(0.111 +/- 0.031) micro mol/mg, (0.143 +/- 0.025) micro mol/mg, (0.183 +/- 0.027) micro mol/mg] were also significantly different (P < 0.01), which was negatively correlated with fluoride concentration in rat hippocampus (r = -0.700, P < 0.01). The activity of butylcholinesterase in high dosage group [(0.041 +/- 0.010) micro mol/mg] was different from that of control [(0.067 +/- 0.025) micro mol/mg, P < 0.05], but the activity was not significantly related with fluoride concentration in rat hippocampus (r = -0.317, P = 0.094).
CONCLUSIONFluoride may go through the blood-brain barrier and accumulate in rat hippocampus, and inhibit the activity of cholinesterase.
