Study of platelet membrane glycoprotein expression in mice with decompression sickness.
- Author:
Guo-zhong WANG
1
;
Chun-jin GAO
;
Huan GE
;
Cheng-qing XIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Platelets; chemistry; Decompression Sickness; blood; Female; Integrin beta3; blood; Mice; P-Selectin; blood; Platelet Endothelial Cell Adhesion Molecule-1; blood
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(2):135-136
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of expression of platelet membrane glycoprotein CD31, CD61 and CD62p in the pathogenesis of decompression sickness.
METHODSMice were randomly divided into decompression sickness group and normal control group. The animals in decompression sickness group were exposed to 600 kPa compressed air for 60 minute, then they were rapidly decompressed to normal pressure in one minute. At 60th minute after reducing to normal pressure, the expression of CD31, CD61 and CD62p on platelet membrane in mice was measured by flow cytometry.
RESULTSThe mean fluorescence intensity of CD31, CD61 and positive percentage of CD62p on platelet membrane [(18.64 +/- 1.01), (271.06 +/- 24.25), (4.48% +/- 0.43%) respectively] in decompression sickness group were significantly increased compared with normal control group [(16.89 +/- 1.69), (234.09 +/- 15.96), (3.00% +/- 0.66%) respectively] (P < 0.05, P < 0.01).
CONCLUSIONInadequately rapid decompression may induce up regulation of platelet membrane glycoprotein CD31, CD61 and CD62p expression in mice, which may lead to thrombosis.
