Inhibitory effect of PD98059 on MAPK signaling pathway in acute lymphocytic leukemia cells.
10.7534/j.issn.1009-2137.2013.06.007
- Author:
Qian-Yu LI
1
;
Xu-Dong WEI
1
;
Lin CHEN
1
;
Qing-Song YIN
2
Author Information
1. Department of Hematopathy, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, Henan Province, China.
2. Department of Hematopathy, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, Henan Province, China. E-mail:jnyinqingsong@163.com.
- Publication Type:Journal Article
- MeSH:
Flavonoids;
pharmacology;
Humans;
MAP Kinase Kinase Kinases;
metabolism;
MAP Kinase Signaling System;
drug effects;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
metabolism;
Proto-Oncogene Proteins c-fos;
metabolism;
Proto-Oncogene Proteins c-jun;
metabolism;
Tumor Cells, Cultured
- From:
Journal of Experimental Hematology
2013;21(6):1399-1402
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the effect of blocking Ras/Erk signaling pathway on expression of important transcription factor c-fos, c-jun and TAK1 gene in primary acute lymphocytic leukemia (ALL) cells. The best effective concentration and effect time of PD98059 were screened; the expression levels of c-fos, c-jun and TAK1 in primary cultured cells of normal persons, primary cultured ALL cells and primary cultured ALL cells treated by PD98059 were detected by SYBR GreenI real-time quantitative-PCR. The results showed that before treatment by PD98059 the expression levels of c-fos and TAK1 mRNA were significantly up-regulated in primary cultured ALL cells as compared with primary cultured cells of normal persons (P = 0.014 and P = 0.017 respectively). After treatment by PD98059, the expression levels of c-fos, c-jun mRNA decreased in all 7 serum samples, while expression of TAK1 was down-regulated in 5 samples, and up-regulated in 2 samples. After treatment with PD98059, there was no statistical difference of c-fos, c-jun and TAK1 expression levels in primary cultured ALL cells and primary cultured normal cells. It is concluded that the c-fos and TAK1 activity of primary cultured ALL cells increases, and blocking the Ras/Erk signaling pathway of ALL cells can lead to obvious decrease of important transcription factors c-fos, c-jun, TAK1 genes expression.
