Effect of salidroside on apoptosis of bone marrow mesenchymal stem cells induced by ara-C.
10.7534/j.issn.1009-2137.2013.06.039
- Author:
Yu-Ping WEI
1
;
Hai BAI
2
;
Yan-Qing SUN
3
;
Shen BAO
1
;
Rui XI
4
;
Lin LIU
4
Author Information
1. Department of Hematology, Ningxia People's Hospital, Yinchuan 750012, Ningxia Province, China.
2. Key Laboratory of Stem cell and Gene Drugs in Gansu Province, Department of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China. E-mail:baihai98@torn.corn.
3. Department of Hematology, Gansu Provincial People's Hospital, Lanzhou 730050, Gansu Province, China. E-mail:yanqingfang40@163.com.
4. Key Laboratory of Stem cell and Gene Drugs in Gansu Province, Department of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Bone Marrow Cells;
cytology;
drug effects;
Cells, Cultured;
Cytarabine;
pharmacology;
Glucosides;
pharmacology;
Humans;
Mesenchymal Stromal Cells;
cytology;
drug effects;
Phenols;
pharmacology
- From:
Journal of Experimental Hematology
2013;21(6):1572-1577
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to investigate the effect of salidroside on human bone marrow mesenchymal stem cell (hBMMSC) apoptosis induced by cytarabine C (Ara-C) and its mechanism, hBMMSC were cultured in vitro and isolated by Fircoll density gradient centrifugation; cell surface antigens were measured by flow cytometry; the osteogenic and adipogenic differentiation of MSC was tested and evaluated by specific staining methods. The proliferation and apoptosis of cells exposed to Ara- C were detected by MTT and flow cytometry respectively. The experiments were divided into 4 groups: control group, Ara-C group, salidroside group and Ara-C+salidroside group. The mRNA expression of BCL-2 and BAX was assayed by RT-PCR. The results showed that the adherent cells displayed spindle and fibroblast cell-like shape; the hBMMSC expressed CD44, CD71 and HLA-ABC, not expressed CD34, CD45 and HLA-DR; the hBMMSC successfully differentiated into osteogenic and adipogenic lineages, which showed mineralization with von Kossa staining. Furthermore, liquid vacuoles were detected by oil red O staining; Ara- C exhibited a less inhibitory effect on the proliferation of hBMMSC treated with salidroside. The apoptosis of hBMMSC treated with salidroside were significantly higher as compared with control group (P < 0.05); RT-PCR results demonstrated that the BCL-2 expression was significantly down regulated but BAX mRNA expressions was up-regulated in Ara- C group as compared with those in the control group. Salidroside significantly inhibited the apoptosis of MSC and reversed the mRNA expression of BCL-2 and BAX. It is concluded that salidroside can inhibit the apoptosis of hBMMSC induced by Ara-C, its mechanism may be related with the regulation of BCL-2/BAX expression.
