MIP-1α promotes the migration ability of Jurkat cell through human brain microvascular endothelial cell monolayer.
10.7534/j.issn.1009-2137.2014.01.008
- Author:
Yi-Ran MA
1
;
Shuang ZHANG
1
;
Ying SUN
1
;
Yi-Yang LIU
1
;
Qian SONG
1
;
Yi-Wen HAO
2
Author Information
1. Department of Blood Transfusion, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
2. Department of Blood Transfusion, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. E-mail: hyw82666@163.com.
- Publication Type:Journal Article
- MeSH:
Brain Neoplasms;
pathology;
secondary;
Cell Adhesion;
Cell Movement;
Chemokine CCL3;
metabolism;
Endothelial Cells;
pathology;
Endothelium, Vascular;
pathology;
Humans;
Jurkat Cells;
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma;
pathology
- From:
Journal of Experimental Hematology
2014;22(1):35-39
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to explore the mechanism of central nervous system (CNS) leukemia resulting from brain metastasis of human acute T-cell leukemia (T-ALL) cells and the role of MIP-1α in migration of Jurkat cells through human brain microvascular endothelial cells (HBMEC). The real-time PCR, siRNA test, transendothelial migration test, endothelial permeability assay and cell adhesion assay were used to detect MIP-1α expression, penetration and migration ability as well as adhesion capability respectively. The results showed that the MIP-1α expression in Jurkat cells was higher than that in normal T cells and CCRF-HSB2, CCRF-CEM , SUP-T1 cells. The MIP-1α secreted from Jurkat cells enhanced the ability of Jurkat cells to penetrate through HBMEC, the ability of Jurkat cells treated by MIP-1α siRNA to adhere to HBMEC and to migrate trans endothelial cells decreased. It is concluded that the MIP-1α secreted from Jurkat cells participates in process of penetrating the Jurkat cells through HBMEC monolayer.
