Prospective study on comparing two methods: for dynamic monitoring of minimal residual disease in acute promyelocytic leukemia.
10.7534/j.issn.1009-2137.2014.01.011
- Author:
Yan ZHANG
1
;
Li-Ping TIAN
1
;
Ying ZHANG
1
;
Ming-Huan DUAN
2
;
Xiao-Ming QIN
3
Author Information
1. Department of Hematology, Beijing Longfu Hospital, 100010 Beijing, China.
2. Department of Hematology, Beijing Union Hospital, Beijing 100730, China. E-mail: mhduan@sina.com.
3. Beijing Hightrust Institnte of Clinical Laboratorial Examination, Beijing 100176, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Female;
Flow Cytometry;
methods;
Humans;
Leukemia, Promyelocytic, Acute;
therapy;
Male;
Middle Aged;
Neoplasm, Residual;
therapy;
Polymerase Chain Reaction;
methods;
Prospective Studies;
Young Adult
- From:
Journal of Experimental Hematology
2014;22(1):50-53
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to comparatively analyse the value of PCR and FCM for dynamic monitoring minimal residual disease (MRD) of acute promyelocytic leukemia. The patients with acute promyelocytic leukemia hospitalized in our hospital from January 2011 to December 2012 were observed and all achieved complete remission after remission induction therapy. Before the chemotherapy, the bone marrow cell morphology examination, polymerase-chain reaction (PCR) and multi-parameter flow cytometry (FCM) were performed for each patient. Then the detection results were statistically analyzed. The 477 specimens were achieved from 159 detections for 48 patients. The results showed that 3 specimens were found to be relapsed by bone marrow cell examination, and other specimens were complete remission;PCR detection confirmed 7 positive, and the FCM confirmed 19 positive. There wasn't significant difference between PCR and FCM by kappa test (P > 0.05). It is concluded that FCM is as sensitive as PCR in evaluating the treatment effect of acute promyelocytic leukemia.
