Expression of miR-16 in patients with T lymphoblastic lymphoma/acute lymphoblastic leukemia.
10.7534/j.issn.1009-2137.2014.01.020
- Author:
Lai-Gen TONG
1
;
Wen-Zhong WU
2
;
Yun-Ping ZHANG
1
;
Zhi-Gang ZHOU
1
;
Ya-Feng CHEN
1
;
Wen-Juan HUANG
1
;
Huan XU
1
;
Qian-Qian SU
1
Author Information
1. Department of Hematology, Yixing Municipal People's Hospital, Yixing, 214200, Jiangsu Province, China.
2. Department of Hematology, Yixing Municipal People's Hospital, Yixing, 214200, Jiangsu Province, China. E-mail: staff324@yxph.com.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Child;
Child, Preschool;
Female;
Humans;
Male;
MicroRNAs;
genetics;
Middle Aged;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
genetics;
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma;
genetics;
Prognosis;
Proto-Oncogene Proteins c-bcl-2;
genetics;
Survival Rate;
Young Adult
- From:
Journal of Experimental Hematology
2014;22(1):99-103
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the expression of miR-16 in T lymphoblastic lymphoma/acute lymphoblastic leukemia (T-LBL/ALL) and its relation with target therapy and prognosis. The CD3, cCD3, CD10, CD20, CD34, CD43, CD99, TdT, PAX-5, BCL-2 and Ki67 in paraffin samples from 38 cases of T-LBL/ALL were detected by immunohistochemical labeling; the miR-16 expression level was detected by real-time RT-PCR. Fifteen cases of reactive hyperplasia of lymph nodes were selected as control. The results indicated that among 38 cases of T-LBL/ALL the positive rate of TdT was highest (94.7%), the positive rate of CD34 was lowest (22.1%), the PAX-5 and CD20 were found to be negative. The Ki67 expression level in 39.5% cases exceeded 80%. As compared with reactive hyperplasia of lymph node, the miR-16 expression in T-LBL/ALL was up-regulated, ant its expression level was 4.87-fold of reactive hyperplasia of lymph node (P < 0.05). The overall survival rate in group of miR-16 high expression decreased (P < 0.05). The prognosis of T-LBL/ALL patients with BCL-2 positive expression was better than that of patients with BCL-2 negative expression (P < 0.05). The miR-16 expression correlated with BCL-2 protein (r = 0.51, P < 0.05). It is concluded that the overall survival rate in miR-16 high expression group is higher than that in miR-16 low expression group, suggesting possible relation of miR-16 with prognosis. Moreover, the prognosis in BCL-2 positive expression group is better than that in negative expression group, which may be a factor influencing prognosis.
