Role of antioxidant in protecting the biological function of hematopoietic stem cells.
10.7534/j.issn.1009-2137.2014.01.028
- Author:
Yi-Wen HAO
1
;
Huan-Ming XU
2
;
Da-Ye CHENG
2
;
Yi-Ran MA
2
;
Li JING
3
Author Information
1. Department of Blood Transfusion, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. E-mail hyw82666@163.com.
2. Department of Blood Transfusion, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
3. Department of Diagnosis and Therapy for Cadres, Shenyang Fourth People's Hospital, Shenyang 110031, Liaoning Province, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
metabolism;
Antioxidants;
pharmacology;
Ascorbic Acid;
analogs & derivatives;
pharmacology;
Cells, Cultured;
Hematopoietic Stem Cells;
cytology;
drug effects;
Humans;
Reactive Oxygen Species;
metabolism
- From:
Journal of Experimental Hematology
2014;22(1):142-147
- CountryChina
- Language:Chinese
-
Abstract:
In peripheral blood hematopoietic stem cell transplantation (PBHSCT) , the mobilization and circulating of bone marrow hematopoietic stem cells in blood with higher oxygen concentration all increase reactive oxygen species(ROS) production, which has negative effect on the biological function of BMHSC. In order to investigate the protective effect of antioxidant on hematopoietic stem cells (HSC), the ascorbic acid 2-phosphate (AA2P), an ascorbic acid derivative of vitamin C, was added in HSC culturing by imitating oxygen conditions which BMHSC experienced in peripheral blood stem cell transplantation. The protective effect of above-mentioned culture methods on the biologic functions of BMHSC was evaluated by vitro amplification assay, committed division assay, reactive oxygen species (ROS) measurement, CD34(+) HSC engraftment. The results showed that the ROS level in HSC from in vitro cultures was much higher than that freshly separated BMHSC, and the amplified AC133(+)CD34(+) HSC, BFU-E, CFU-GM, CFU-GEMM colonies, migration rate and severe combined immunodeficiency (SCID)-repopulating cells (SRC) were all much more than HSC cultured without AA2P. It is concluded that antioxidant intervention may be an effective methods for protecting the biological function of PBHSC and improving the therapeutic effect of PBHSCT.
