Optimization for extraction technology of polysaccharide in root of Adenophora potaninii from Taibai mountain in China by orthogonal experimental design.
- Author:
Peng-Cheng LI
1
;
Ben-Cai ZHANG
;
Zhan-Guo CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Campanulaceae; chemistry; China; Drugs, Chinese Herbal; chemistry; Plant Roots; chemistry; Polysaccharides; isolation & purification; Temperature; Time Factors; Water; chemistry
- From: China Journal of Chinese Materia Medica 2008;33(11):1266-1312
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study is to develop excellent extraction technology of the polysaccharides in the root of the A. potaninii which live in the Taibai Mountain in China.
METHODBased on the extraction with water, the polysaccharides were deposited with alcohol. With the content of polysaccharides was as the index, extraction conditions were investigated systemly. Employed the solid-liquid ratio, extraction temperature, extraction time and extraction number of times were as levels of single factor, the optimal extraction technology of the polysaccharides from the root of the A. potaninii was determined by L9 (3(4)) orthogonal experimental design L9 (3(4)).
RESULTThe optimal technology conditions were that the solid-liquid ratio was 1 : 30, the extracting temperature was 60 degrees C, the extracting time was 3 h and extracting number of times was 3 times.
CONCLUSIONThe optimized extraction technology is simple, reliable and extraction efficiency of polysaccharide is higher.
