Monosaccharide composition analysis and its content determination of polysaccharides from Rhaponticum uniforum.
- Author:
Fa-Sheng LI
1
;
Heng-Gui XU
;
Xiao-Mei YAN
;
Ming-Yang LI
;
Hui LIU
Author Information
- Publication Type:Journal Article
- MeSH: Calibration; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Leuzea; chemistry; Monosaccharides; analysis; isolation & purification; Polysaccharides; analysis; isolation & purification; Reproducibility of Results; Sensitivity and Specificity
- From: China Journal of Chinese Materia Medica 2008;33(11):1284-1286
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the monosaccharide composition in the polysaccharides from Rhaponticum uniforum, determine the content of monosaccharide, and provide some references for further research.
METHODThe monosaccharide composition was determined by high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Phenol-sulfuric acid method was used for the determination of the content of polysaccharide.
RESULTThe monosaccharides composition in polysaccharides from R. uniforum are glucose, arabonose and fructose. Their molar ratios are 1 : 1.61 : 2.21. The content of polysaccharide is 95.78%, taking the mixture of monosaccharide compositions as reference substances.
CONCLUSIONHPAEC-PAD can be used to analyze the monosaccharide composition in the polysaccharide with high precision, and the method of phenol-sulfuric acid is simple, convenient and reliable.
