Determination of plasma protein binding rate of methyl protodioscin with ultrafiltration.
- Author:
Zhi-Hong YAO
1
;
Xiu-Zhen CAO
;
Meng SHAO
;
Yu-Ming PAN
;
Wen-Cai YE
;
Xin-Sheng YAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents; metabolism; Blood Proteins; metabolism; Calibration; Chromatography, High Pressure Liquid; Diosgenin; analogs & derivatives; metabolism; Female; Humans; Male; Protein Binding; Rats; Saponins; metabolism; Sensitivity and Specificity; Tandem Mass Spectrometry; Ultrafiltration
- From: China Journal of Chinese Materia Medica 2008;33(11):1291-1294
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the plasma protein binding rate of methyl protodioscin.
METHODThe ultrafiltration was employed to determine the plasma protein binding rate of methyl protodioscin. The plasma concentrations of methyl protodioscin were measured by HPLC-MS-MS.
RESULTThe plasma protein binding rate of methyl protodioscin with rat plasma at the concentration of 20.0, 100 and 200 microg x mL(-1) were (94.6 +/- 0.16)%, (91.6 +/- 0.35)% and (86.10 +/- 0.60)%, respectively, while the plasma protein binding rate of methyl protodioscin with normal human plasma at the above concentrations were (82.11 +/- 5.12)%, (84.54 +/- 0.32)% and (88.52 +/- 1.02)%, respectively.
CONCLUSIONThe binding rate of methyl protodioscin with plasma protein is high.
