Effects of Armillariella tabescens polysaccharide IPS-B2 on activity of mouse peritoneal macrophages and transcription of related gene.
- Author:
Xia LUO
1
;
Meng-Yao YU
;
Xiao-Yan XU
;
Jin ZENG
;
Nan JIANG
;
Lin-Yong ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Agaricales; chemistry; Animals; Gene Expression Regulation; drug effects; Interleukin-1beta; biosynthesis; genetics; Interleukin-6; biosynthesis; genetics; Macrophages, Peritoneal; drug effects; metabolism; Mice; Nitric Oxide; biosynthesis; Nitric Oxide Synthase Type II; metabolism; Polymerase Chain Reaction; Polysaccharides; pharmacology; RNA, Messenger; biosynthesis; Transcription, Genetic; drug effects; Tumor Necrosis Factor-alpha; biosynthesis; genetics
- From: China Journal of Chinese Materia Medica 2008;33(11):1305-1308
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of IPS-B2 on mouse peritoneal macrophages and the transcription of IL-1beta, IL-6, TNF-alpha and iNOS.
METHODELISA method and Griess method were used to detect the effect of mouse peritoneal macrophages produce cytokines IL-1beta, IL-6, TNF-alpha and cytotoxic effectors NO. The transcription of IL-1beta, IL-6, TNF-alpha and iNOS was detected by real-time RT-PCR method.
RESULTIPS-B2 could not promote mouse peritoneal macrophage production, but it could significantly improve the IL-1beta, IL-6, TNF-alpha content in mouse peritoneal macrophages culture supernatant, and increase the gene expression of IL-1beta, IL-6, TNF-alpha and iNOS.
CONCLUSIONIPS-B2 can enhance the ability of peritoneal macrophages to excrete bioactive substances and promote the transcription of bioactive substances to antitumor.
