Chemical constituents and anti-tuberculosis activity of root of Rhodiola kirilowii.
- Author:
Ying-Chun WONG
1
;
Ming ZHAO
;
Yu-Ying ZONG
;
Chiu-Yeung CHAN
;
Chun-Tao CHE
Author Information
1. School of Chinese Medicine, Chinese University of Hong Kong, Hong Kong, China.
- Publication Type:Journal Article
- MeSH:
Antitubercular Agents;
isolation & purification;
pharmacology;
Magnetic Resonance Spectroscopy;
Mass Spectrometry;
Microbial Sensitivity Tests;
Mycobacterium tuberculosis;
drug effects;
Plant Roots;
chemistry;
Rhodiola;
chemistry
- From:
China Journal of Chinese Materia Medica
2008;33(13):1561-1565
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents of Rhodiola kirilowii were separated and purified by repeated column chromatography on silica gel, RP - 18, Sephadex LH -20 and semi-preparative HPLC. Each compound was characterized by spectroscopic and physical data. Twelve compounds have been purified and identified to be beta-sitosterol (1), tyrosol (2), trans-hydroxycinnamic acid (3), geranyl beta-glucopyranoside (4), neryl beta-glucopyranoside (5), hexyl beta-glucopyranoside (6), gallic acid (7), (-) -epigallocatechin gallate (8), rhodiolgin (9), isolariciresinol-9-O-beta-glucopyranoside (10), rhodiooctanoside (11), and sacranoside B (12). Among them, compounds 3, 6, 9-12 were isolated from Rhodiola kirilowii for the first time; Compounds 4 and 5 were obtained for the first time from the genus Rhodiola. The in vitro activities against Macobacterium tuberculosis (ATCC 27294) of its extracts and pure components were evaluated by testing their MIC (minimal inhibitory concentration) and MBC (minimal bactericidal concentration). The 80% (a. q.) EtOH extract, EtOAc-soluble extract, compounds 7 and 8 exhibited in-vitro inhibitory and bactericidal activities against Macobacterium tuberculosis in different extent.
