Study on isozymes in six species of Curcuma.
- Author:
Jia-yong TANG
1
;
Qing-miao LI
;
Rui-wu YANG
;
Jin-qiu LIAO
;
Yon-hong ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Cluster Analysis; Curcuma; classification; enzymology; genetics; Electrophoresis, Polyacrylamide Gel; Esterases; analysis; genetics; Isoenzymes; analysis; genetics; Peroxidase; analysis; genetics; Phylogeny; Species Specificity
- From: China Journal of Chinese Materia Medica 2008;33(12):1381-1386
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore genetic relationships of the 39 materials in six species of Curcuma.
METHODThe peroxidase isozyme (POD) and esterase isozyme (EST) were studied using vertical slab polyacrylamide gel electrophoresis (PAGE) technique, and the zymograms were analyzed using the software of NTSYSpc2. 1.
RESULTThe interspecific zymogramatic differences were obvious. Each species possessed its own specific zymogram distinguishing form the others. In the analysis of EST isozyme, C. phaeocaulis, C. wenyujin, C. kwangsiensis and C. chuanhuangjiang had their own specific zymogram. In the analysis of POD isozyme, just C. phaeocaulis and C. kwangsiensis had their specific zymogram.
CONCLUSIONThe genetic relationships are not associated with the geographical distributions and the genetic relationship between C. longa and C. sichuanensis are very close.
