Study on absorption mechanism of genistein self-microemulsifying system in rat intestines.
- Author:
Xian-hua DU
1
;
Xin NIU
;
Qian-jin FENG
;
Hong DU
;
Hai-yan LI
Author Information
- Publication Type:Journal Article
- MeSH: ATP Binding Cassette Transporter, Sub-Family B; antagonists & inhibitors; Animals; Chromatography, High Pressure Liquid; Emulsions; Genistein; analysis; metabolism; pharmacokinetics; Hydrogen-Ion Concentration; Intestinal Absorption; drug effects; Intestines; drug effects; metabolism; Male; Organ Specificity; Rats; Rats, Wistar; Temperature; Verapamil; pharmacology
- From: China Journal of Chinese Materia Medica 2008;33(12):1406-1409
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the absorption mechanism of genistein self-microemulsifying system in rat intestines.
METHODThe concentrations of phenol red and genistein by in situ perfusion in rats were determined by UV and HPLC, respectively. The effects of drug concentrations, pH, various intestinal segments and P-glycoprotein (P-gp) inhibitor verapamil on the absorption had been studied.
RESULTThe absorption rate constant (Ka) of genistein had no significant difference at concentrations of 0.05-0.5 mg x mL(-1) and pH of 5.4-7.8 in perfusion. It was Ka of jejunum > ileum > duodenum > colon. The absorption of genistein in jejunum had significant difference (P < 0.05) compared with other parts of intestines. Ka was increased obviously when verapamil was coper-fused with genistein (P < 0. 05).
CONCLUSIONThe absorption of genistein self-microemulsifying system is a first order process with passive diffusion mechanism related to P-gp efflux. It can be absorbed at all segments of rat intestine, and the jejunum is the best absorption segment, pH had no special effect on the absorption of genistein self-microemulsifying system in rat intestine.
