Reversal effect of aloe emodin liposomes on cisplatin resistance line A549/DDP human lung adenocarcinoma cells.
- Author:
Rong ZENG
1
;
Zhi-wu ZHOU
;
Cui-fang WU
;
Yu-lu ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; pathology; Animals; Anthraquinones; adverse effects; chemistry; pharmacology; Cell Line, Tumor; Cisplatin; pharmacology; Drug Resistance, Multiple; drug effects; Drug Resistance, Neoplasm; drug effects; Humans; Inhibitory Concentration 50; Lethal Dose 50; Liposomes; Lung Neoplasms; pathology
- From: China Journal of Chinese Materia Medica 2008;33(12):1443-1445
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the reversal effect of aloe emodin liposomes (AE-L) on cisplatin resistance human lung adenocarcinoma cell line A549/DDP.
METHODThe un-cytotoxic and cytotoxic concentration of AE-L, and un-cytotoxic concentration of E-L were determined by MTT. The sensitivity of cisplatin were determined by MTT assay in above 3 groups. The intracellular concentration of cisplatin was detected by inductively coupled plasma mass spectrometry (ICP-MS).
RESULTThe maximum non-toxic concentration group of AE-L (2.0 mg x L(-1)) increased the sensitivity of cisplatin in A549/DDP, decreased IC50 of cisplatin in A549/DDP from 16.81 mg x L(-1) to 5.86 mg x L(-1), and the hyp-cytotoxic concentration (7.0 mg x L(-1) ) group's IC50 decreased to 4.34 mg x L(-1); AE-L groups significantly increased intracellular concentration of cisplatin in A549/DDP cells.
CONCLUSIONThe results showed that aloe emodin can reverse multidrug resistance (MDR) of A549/DDP cells and the mechanism might be associated with the increase of intracellular concentration of cisplatin.
