Studies on chemical constituents from rhizome of Anemone flaccida.
- Author:
Lan-tian ZHANG
1
;
Yoshihisa TAKAISHI
;
Yan-wen ZHANG
;
Hong-quan DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Anemone; chemistry; Antineoplastic Agents; chemistry; pharmacology; Cell Proliferation; drug effects; Drugs, Chinese Herbal; chemistry; pharmacology; Eleutherococcus; chemistry; Glycosides; chemistry; pharmacology; HeLa Cells; Humans; Magnetic Resonance Spectroscopy; Oleanolic Acid; chemistry; pharmacology; Plant Extracts; chemistry; pharmacology; Rhizome; chemistry; Spectrometry, Mass, Electrospray Ionization
- From: China Journal of Chinese Materia Medica 2008;33(14):1696-1699
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the chemical constituents from Anemone flaccida.
METHODChemical constituents were isolated by repeated column chromatography (silica gel, Toyopearl HW-40C and preparative HPLC). The structures were elucidated on the basis of spectral data analysis.
RESULTTwelve triterpenes were isolated and their structures were identified as follow: oleanolic acid (1), oleanolic acid 3-O-beta-D-glccopyranosyl-(1-->2)-beta-D-xylopyranoside (2), eleutheroside K (3), oleanolic acid 3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-xylopyranoside (4), oleanolic acid 3-O-beta-D-glccopyranosyl-(1-->2)-alpha-L-arabinofurnoside (5), oleanolic acid 3-O-beta-D-glccuronopyranose (6), oleanolic acid 3-O-beta-D-glccuronopyranose methyl ester (7), oleanolic acid 28-O-alpha-L-rhamnopyranosyl(1-->4)-beta-D-glccopyranosyl (1-->6)-beta-D-glccopyranosyl (8), oleanolic acid 3-O-beta-D-glccuronopyranose 28-O-alpha-L-rhamnopyranosyl (1-->4)-beta-D-glccopyranosyl (1-->6)-beta-D-glccopyranoside (9), oleanolic acid 3-O-beta-D-glccopyranosyl methyl ester 28-O-alpha-L-rhamnopyranosyl (1-->4)-beta-D-glccopyranosyl (1-->6)-beta-D-glccopyranoside (10), oleanolic acid 3-O-beta-D-glccopyranosyl-(1-->2)-beta-D-xylopyranosyl-28-O-alpha-L-rhamnopyranosyl (1-->4)-beta-D-glccopyranosyl (1-->6)-beta-D-glccopyranoside (11), oleanolic acid 3-O-alpha-L-rh-amnopyranosyl-(1-->2)-alpha-L-arabinopyrnosyl-28-O-alpha-L-rhamnopyranosyl (1-->4)-beta-D-glccopyranosyl (1-->6)-beta-D-glccopyranoside (12).
CONCLUSIONcompounds 5-8, 10, 12 were isolated from this plant for the first time. Compounds 2, 5 and 11 showed positive anti-tumor activities.
