Coumarins from branch of Fraxinus sieboldiana and their antioxidative activity.
- Author:
Sheng LIN
1
;
Ming-tao LIU
;
Su-juan WANG
;
Shuai LI
;
Yong-chun YANG
;
Jian-gong SHI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antioxidants; chemistry; pharmacology; Coumarins; chemistry; pharmacology; Fraxinus; chemistry; Lipid Peroxidation; drug effects; Magnetic Resonance Spectroscopy; Microsomes, Liver; drug effects; metabolism; Rats; Scopoletin; chemistry; pharmacology; Spectrometry, Mass, Electrospray Ionization; Umbelliferones; chemistry; pharmacology
- From: China Journal of Chinese Materia Medica 2008;33(14):1708-1710
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the chemical constituents from the branch of Fraxinus sieboldiana, and evaluate their antioxidative activity.
METHODThe chemical constituents were isolated and purified by chromatographic techniques over silica gel, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC. Structures of the compounds were identified by spectroscopic methods. The antioxidant activity was evaluated by Fe(+2)-cystine induced rat liver microsomal lipid peroxidation.
RESULTEight coumarins were obtained and their structures were elucidated as esculin (1) , esculetin (2), fraxin (3), fraxetin (4), 6, 7-di-O-beta-D-glucopyranosylesculetin (5), scopoletin (6), cleomiscosin D (7) and cleomiscosin B (8). At a concentration of 10(-6) mol x L(-1), compound 4 showed antioxidative activity inhibiting Fe(+2)-cystine induced rat liver microsomal lipid peroxidation with inhibitory rate of 60%.
CONCLUSIONCompounds 5, 7 and 8 were obtained from the genus Fraxinus for the first time. Compound 4 showed remarkable antioxidative activity, which was higher than that of VE (35%).
