The study of pro-nucleating activity on 33.5 x 10(3) vesicular protein in bile.
- Author:
Baojin MA
1
;
Jianbin XIANG
;
Duan CAI
;
Yanling ZHANG
;
Xiliang ZHA
;
Liying WANG
Author Information
- Publication Type:Journal Article
- MeSH: Bile; metabolism; Cholelithiasis; physiopathology; Cholesterol; metabolism; Humans; Macromolecular Substances; Microscopy; Models, Biological; Protein Transport; Proteins; metabolism
- From: Chinese Journal of Surgery 2002;40(4):298-300
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the pro-nucleating activity in 33.5 x 10(3) vesicular protein.
METHODSThe model biles were established according Kibe and Zhu. The pro-nucleating activity of 33.5 x 10(3) vesicular protein were examined by polarized light microscopy. The protein and its enzymatic deglycosylation and proteolysis fractions nucleation promoting activity were detected by cholesterol crystal growth assay.
RESULTS33.5 x 10(3) vesicular protein displayed apparent potency of pro-nucleation with activity of 0.310, and derived crystal growth curve indices It, Ig, Ic were presented as 0.57, 1.52, 1.63 respectively, but after treated by N-glycanase enzyme and pronase, no promoting activity were found.
CONCLUSIONThe 33.5 x 10(3) vesicular protein may be involved in the nucleation process of gallstone formation, which is regulated by its peptide and sugar chain.
