Expression and significance of bcl-2 and bcl-xL in rat neuron after acute brain trauma.
- Author:
Xinyu YANG
1
;
Shuyuan YANG
;
Jianning ZHANG
;
Liang XUE
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Animals; Apoptosis; Brain Injuries; metabolism; pathology; Immunohistochemistry; Male; Neurons; chemistry; pathology; Proto-Oncogene Proteins c-bcl-2; analysis; Rats; Rats, Wistar; bcl-X Protein
- From: Chinese Journal of Surgery 2002;40(9):702-704
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expression of bcl-2 and bcl-xL in rat brain neuron after acute brain trauma.
METHODSThe rat model of mild and sever brain trauma were made by diffused brain injury. The expression of bcl-2 and bcl-xL in rat brain neuron was examined by immunohistochemical staining. Rat neuron apoptosis was detected by TUNEL method.
RESULTSThere were few bcl-2 (cortex: 4.40 +/- 1.67, hippocampal: 3.20 +/- 1.30) and lots of bcl-xL (cortex: 45.60 +/- 4.34, hippocampal: 50.20 +/- 3.50) expression in normal controls. After impact, the expression of bcl-2 in rat brain neuron increased, most distinctly on day 1(cortex: 30.0 +/- 4.3, hippocampal: 46.6 +/- 3.2), in mild group. A negative correlation was seen between bcl-2 expression and neuronal apoptosis (-1 < r < -0.847, P< 0.01, n = 10). No change was seen in bcl-xL expression.
CONCLUSIONSbcl-2 expression increased after acute brain trauma but bcl-xL did not change much. Both bcl-2 and bcl-xL are concerned with anti-apoptosis in neuron after acute brain trauma.
