Expression of core-binding factor a1 by human skin fibroblasts induced in vitro.
- Author:
Lianfu DENG
1
;
Wei FENG
;
Yue ZHANG
;
Yaping ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Bone Morphogenetic Protein 2; Bone Morphogenetic Protein Receptors, Type I; Bone Morphogenetic Proteins; pharmacology; Cells, Cultured; Collagen; biosynthesis; Core Binding Factor Alpha 1 Subunit; Core Binding Factors; Fibroblasts; metabolism; Humans; Neoplasm Proteins; Osteocalcin; biosynthesis; Protein-Serine-Threonine Kinases; biosynthesis; RNA, Messenger; analysis; Receptors, Growth Factor; biosynthesis; Skin; cytology; Transcription Factors; biosynthesis; genetics; Transforming Growth Factor beta; Tumor Necrosis Factor-alpha; pharmacology
- From: Chinese Journal of Surgery 2002;40(8):592-595
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the probabilities of core-biding factor a1 (Cbfa1) expression by human skin fibroblasts induced in vitro.
METHODSThe fibroblasts were isolated, purified from human skin, and were grown in incubation in the media of TNF-alpha, BMP-2, and combined TNF-alpha and BMP-2 at certain concentrations, respectively. The changes in biological features of these fibroblasts correlated with osteogenesis were detected by immunohistochemistry and RT-PCR assay.
RESULTSTNF-alpha could switch phenotype of collagen in fibroblasts from Type I and III to Type I and induce fibroblasts to express Ras and BMP type I receptor (BMPR-IA). TNF-alpha in combination with BMP-2 could induce fibroblasts to express Cbfa1 and osteocalcin mRNA.
CONCLUSIONHuman skin fibroblast could be induced into pro-osteoblast expressing Cbfa1, an osteoblast-specific transcription factor and a regulation of osteoblast differentiation, and combined use of TNF-alpha and BMP-2 was one of the regulating factors.
