Effects of cartilage-derived growth factor on cultured rabbit chondrocytes.
- Author:
Jinfeng MA
1
;
Xiaoliang CHEN
;
Yingzhen WANG
;
Jixian WANG
;
Hongshi HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cartilage; metabolism; Cell Division; drug effects; Chondrocytes; drug effects; physiology; Dose-Response Relationship, Drug; Drug Synergism; Hydroxyproline; biosynthesis; Insulin-Like Growth Factor I; pharmacology; Male; Proteins; pharmacology; Rabbits
- From: Chinese Journal of Surgery 2002;40(8):600-603
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of cartilage-derived growth factor (CDGF) on cultured rabbit chondrocytes, and the relation between CDGF, insulin-like growth factor-I (IGF-I), and proliferation and metabolism of chondrocytes.
METHODSCDGF was extracted from chicken xiphoid in our laboratory. Chondrocytes were isolated from 3-week-old New Zealand male rabbits, and cultured in Ham's F-12 medium containing 10% fetal calf serum. The chondrocyts of second generation were treated with CDGF and/or IGF-I of different concentrations. With chloramine T method and MTT method, we compared the content of hydroxyproline in Ham's F-12 medium and observed proliferation and energy synthesis of chondrocytes.
RESULTSCDGF and IGF-I both stimulated the proliferation and synthesis of hydroxyproline of chondrocytes dose-dependently. The optimal concentration of CDGF was 16 ng/ml and 32 ng/ml respectively, and that of IGF-I was 30 ng/ml. There was obvious synergic effect between CDGF and IGF-I.
CONCLUSIONCDGF can stimulate the proliferation and collagen synthesis of chondrocytes and has synergistic effect with IGF-I.
