Enhancement of ionizing radiation on liposome-mediated gene delivery in human rectal cancer HR-8348 cells.

Author: Wenping LU ¹ ; Shiyong LI ; Ping AN ; Bo YU
Author Information

1. Department of General Surgery, Beijing Military Area General Hospital, Beijing 100700, China.
Publication Type:Journal Article
MeSH: Dose-Response Relationship, Radiation; Gene Transfer Techniques; Genetic Therapy; Humans; Liposomes; Rectal Neoplasms; therapy; Transfection
From: Chinese Journal of Surgery 2002;40(8):604-605
CountryChina
Language:Chinese
Abstract:
OBJECTIVESTo investigate the effect of ionizing radiation on liposome-mediated gene delivery and find out a way to improve gene transfection.
METHODSPrior to liposome transfection, HR-8348 cells were irradiated at doses of 0, 2, 4, 8 Gy selected according to the surviving fraction line of HR-8348 cells after different dosage of radiation. After 36 h of liposome transfection, green fluorocytes were counted. The transfection efficiency was figured out and compared with each other.
RESULTSThe transfection efficiency of liposome-mediated gene delivery was 21.32%, 62.17%, 68.00%, 77.78% at the dose of 0, 2, 4, 8 Gy respectively and the clinical dose (2 Gy) was as high as 62.17%. Combined radiation and liposome-mediated gene delivery achieved the approximate transfection efficiency of virus vector.
CONCLUSIONIonizing radiation can improve the transfection efficiency of liposome-mediated gene delivery markedly and it is expected to treat human malignancy with liposome-mediated gene delivery combined with radiation.