Establishment and characterization of human extrahepatic growing hepatocellular carcinoma cell line EGHC-9901.
- Author:
Xiaopeng WU
1
;
Zhanmin WANG
;
Bo LIU
;
Jun LIU
;
Yingmao GAO
;
Zhaoting LI
;
Chunsheng LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Animals; Carcinoma, Hepatocellular; genetics; pathology; Chromosome Aberrations; Humans; Liver Neoplasms; genetics; pathology; Male; Mice; Mice, Nude; Tumor Cells, Cultured; alpha-Fetoproteins; analysis
- From: Chinese Journal of Surgery 2002;40(8):616-617
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a new extrahepatic growing hepatocellular carcinoma cell line.
METHODSA specimen from extrahepatic growing hepatocellular carcinoma was cultured in vitro. Cancer cells were studied morphologically and subjected to karyotype analysis, DNA analysis, and tumor formation evaluation.
RESULTSMorphological observation and functional analysis showed that their features were similar to those of HCC. Chromosomes with a variation of 76 approximately 104 were able to secret AFP in vitro and to form bile canaliculi with microvilli.
CONCLUSIONEGHC-9901 cell line has characteristics of the extrahepatic growing hepatocellular carcinoma.
