Adenovirus-mediated transfer of the herpes simplex virus thymidine rinase gene used by several methods.
- Author:
Tao HUANG
1
;
Guodong GAO
;
Siyuan CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Gene Transfer Techniques; Genetic Therapy; methods; Humans; Liver Neoplasms, Experimental; therapy; Mice; Mice, Inbred BALB C; Simplexvirus; enzymology; Thymidine Kinase; genetics; Tumor Cells, Cultured
- From: Chinese Journal of Surgery 2002;40(8):625-627
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the therapeutic effects of adenovirus-mediated transfer of the herpes simplex virus thymidine rinase gene (HSV-tk) used by several methods and the dose-effect relationship.
METHODSDiverse doses (1 x 10(9) PFU, 1 x 10(10) PFU, 1 x 10(11) PFU) of adenovirus-mediated transfer of HSV-tk were given by intraparenchymatous, intravenous and intraperitoneal injection, and ganciclovir (GCV) (100 mg.kg(-1).d(-1)) was injected into the cavity of the peritoneum to treat human hepatocarcinoma. The change of tumors size was observed and the fragments of HSV-tk gene were tested.
RESULTSIn nude mice after intraparenchymatous injection and high-dose (1 x 10(11) PFU) intravenous injection, the tumors were suppressed significantly (t = 13.1, 12.4, P < 0.01) and lots of fragments of HSV-tk gene were observed. In mice after intraperitoneal injection and low-dose (1 x 10(9) PFU, 1 x 10(10) PFU) intravenous injection, no suppressive effect was observed (t = 1.8, 1.0, 2.1, 1.1, 0.8, P > 0.05) with few or without fragments in the tumors.
CONCLUSIONSAdenovirus-mediated transfer of the HSV-tk by intraparenchymatous or intravenous injection is effective in treatment of hepatocarcinoma in nude mice, but intraperitoneal injection has no therapeutic effect.
