Microarray in screening for differentially expressed genes of cellular cycle and apoptosis in abdominal aortic aneurysms.

Yuehong ZHENG; Heng GUAN; Yongjun LI; Changwei LIU; Bao LIU; Qi SHENG; Shiying MIAO

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Microarray in screening for differentially expressed genes of cellular cycle and apoptosis in abdominal aortic aneurysms.

Author: Yuehong ZHENG ¹ ; Heng GUAN ; Yongjun LI ; Changwei LIU ; Bao LIU ; Qi SHENG ; Shiying MIAO
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1. Department of Vascular Surgery, Beijing Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
Publication Type:Journal Article
MeSH: Aortic Aneurysm, Abdominal; genetics; pathology; Apoptosis; physiology; Cell Cycle; genetics; Cells, Cultured; Gene Expression; Gene Expression Profiling; Humans; Myocytes, Smooth Muscle; metabolism; pathology; Oligonucleotide Array Sequence Analysis
From: Chinese Journal of Surgery 2002;40(11):817-819
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo analyze the functions of differentially expressed genes between abdominal aortic aneurysm and normal aortic tissue by cDNA microarray.
METHODSTotal RNAs were respectively isolated from the normal aorta and aortic aneurysm, purified into mRNAs by oligotex. Subsequently they were reverse-transcribed into cDNAs incorporated with fluorescent dUTP to make hybridization probes, which were hybridized as the cDNA microarray for scanning of fluorescent signals and differentially expressed genes between the normal aortic and aortic aneurysm by using GenePix Pro 3.0 software.
RESULTSA total of 18 differentially expressed genes were detected, accounting for 0.44% of total genes. Among these genes, 11 were related to cell cycle and the remaining 7 to cell apoptosis. The number of upregulated genes in the aortic aneurysm was 9 (mean ratio: 3.860) and that of the downregulated 9 (mean Ratio: 0.294). Bio-informative analysis showed that these 18 genes might influence the growth and apoptosis of smooth muscle cells in abdominal aortic aneurysms.
CONCLUSIONSDuring the development of abdominal aortic aneurysms, modulations of multi-gene expression would undergo various changes. Cell cycle and apoptosis-related genes were related to the growth and apoptosis of smooth muscle cells in abdominal aortic aneurysms. Further research into these genes will clarify the mechanisms of abdominal aortic aneurysms.