Expression of the tyrosine kinase receptor EphA5 and its ligand ephrin-A5 during mouse spinal cord development.

Author: Christopher P WASHBURN ¹ ; Margaret A COOPER ; Renping ZHOU
Author Information

1. Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854-8020, USA.
Publication Type:Journal Article
MeSH: Animals; Ephrin-A5; biosynthesis; Gene Expression; Gene Expression Regulation, Developmental; Immunohistochemistry; Mice; Mice, Inbred C57BL; Mice, Mutant Strains; Receptor, EphA5; biosynthesis; Spinal Cord; embryology; metabolism
From: Neuroscience Bulletin 2007;23(5):249-255
CountryChina
Language:English
Abstract:
OBJECTIVESTo study the expression patterns of two Eph family molecules, the receptor EphA5, and the ligand ephrin-A5, during spinal cord development.
METHODSThe receptor expression was analyzed using beta-galactosidase knockin mice, and affinity ligand probe binding. The ligand expression was assessed using two different affinity probes, and knockout mouse tissues as controls.
RESULTSEphA5 was expressed in the ventral spinal cord, while ephrin-A5 was located in the dorsolateral regions of the spinal cord throughout development.
CONCLUSIONSThese results show that EphA5 and ephrin-A5 are expressed over broad developmental stages and may play important roles in establishing the dorsoventral organization of the spinal cord.