Deglycosylation altered the gating properties of rNav1.3: glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel.
- Author:
Qing XU
1
;
Hui-Wen CHENG
;
Hui-Qiong HE
;
Zhi-Rui LIU
;
Ming HE
;
Hong-Tian YANG
;
Zhi-Lei ZHOU
;
Yong-Hua JI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Electric Conductivity; Electric Stimulation; Gene Transfer Techniques; Glycosylation; drug effects; Homeostasis; drug effects; physiology; Ion Channel Gating; drug effects; physiology; Membrane Potentials; drug effects; physiology; NAV1.3 Voltage-Gated Sodium Channel; Nerve Tissue Proteins; physiology; Oocytes; Patch-Clamp Techniques; Sodium Channels; physiology; Static Electricity; Tunicamycin; pharmacology; Xenopus
- From: Neuroscience Bulletin 2008;24(5):283-287
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo examine the effect of deglycosylation on gating properties of rNav1.3.
METHODSrNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control.
RESULTSCompared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV.
CONCLUSIONGlycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity.
