Controlling the release of bFGF from silk fibroin membrane.

Ya Wei JI; Yan Kong; Ya Hong Zhao; Ya Ling Wang; Jing Zhao; Lu Zhong Zhang; Yu Min Yang

Return

Controlling the release of bFGF from silk fibroin membrane.

Author: Ya Wei JI ^{1
,

2} ; Yan KONG ¹ ; Ya Hong ZHAO ¹ ; Ya Ling WANG ³ ; Jing ZHAO ⁴ ; Lu Zhong ZHANG ¹ ; Yu Min YANG ¹ ;
Author Information

1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China
2. The Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong University, Nantong 226001, Jiangsu, China.
3. College of Chemistry and Chemical Engineering, Nantong University, Nantong 226001, Jiangsu, China.
4. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China.
Publication Type:Letter
MeSH: Animals; Cell Culture Techniques; Fibroblast Growth Factor 2; chemistry; Fibroins; chemistry; PC12 Cells; Rats
From: Biomedical and Environmental Sciences 2014;27(12):973-977
CountryChina
Language:English
Abstract: Since neurotrophic factor is easy to degrade and aggregate, it usually has a short half-life in vitro. To overcome this shortage, neurotrophic factor has been combined with the silk fibroin (SF) membrane to realize less degradation, optimal loading efficiency, sustained release, and good adsorption. By optimizing its binding conditions, main parameters were investigated and its optimal loading efficiency was obtained. bFGF was combined to SF membrane by layer by layer (LbL) static adsorption technique. The natural and nontoxic chondroitin sulfate (CS) was used as a crosslinking agent. Optimization was carried out in three aspects: the concentration of bFGF, the concentration of CS, and the reaction time. This experiment provides a better environment for the growth of cells and offers a new kind material of absorbing neurotrophic factor to meet increasing demand for biological materials.