In vitro and in vivo evaluation of the developed PLGA/HAp/Zein scaffolds for bone-cartilage interface regeneration.

Yong Xin Lin; Zhi Yong Ding; Xiao Bin Zhou; Si Tao LI; De Ming Xie; Zhi Zhong LI; Guo Dong Sun

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In vitro and in vivo evaluation of the developed PLGA/HAp/Zein scaffolds for bone-cartilage interface regeneration.

Author: Yong Xin LIN ¹ ; Zhi Yong DING ² ; Xiao Bin ZHOU ¹ ; Si Tao LI ³ ; De Ming XIE ⁴ ; Zhi Zhong LI ¹ ; Guo Dong SUN ¹
Author Information

1. Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong, China.
2. Department of Traumatology, HuiZhou Municipal Central Hospital, Huizhou 516000, Guangdong, China.
3. Department of Pediatrics, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, Guangdong, China.
4. Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510630, Guangdong, China.
Publication Type:Journal Article
Keywords: Cartilage tissue engineering; Chondrocyte; Electrospun; PLGA/HAp/Zein grafts; hUC-MSCs
MeSH: Animals; Biocompatible Materials; Bone Development; physiology; Cartilage; growth & development; Cells, Cultured; Durapatite; chemistry; Female; Humans; Lactic Acid; chemistry; Male; Mesenchymal Stromal Cells; physiology; Polyglycolic Acid; chemistry; Regeneration; physiology; Tissue Scaffolds; chemistry; Young Adult; Zein; chemistry
From: Biomedical and Environmental Sciences 2015;28(1):1-12
CountryChina
Language:English
Abstract:
OBJECTIVETo investigate the effect of electronspun PLGA/HAp/Zein scaffolds on the repair of cartilage defects.
METHODSThe PLGA/HAp/Zein composite scaffolds were fabricated by electrospinning method. The physiochemical properties and biocompatibility of the scaffolds were separately characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), and fourier transform infrared spectroscopy (FTIR), human umbilical cord mesenchymal stem cells (hUC-MSCs) culture and animal experiments.
RESULTSThe prepared PLGA/HAp/Zein scaffolds showed fibrous structure with homogenous distribution. hUC-MSCs could attach to and grow well on PLGA/HAp/Zein scaffolds, and there was no significant difference between cell proliferation on scaffolds and that without scaffolds (P>0.05). The PLGA/HAp/Zein scaffolds possessed excellent ability to promote in vivo cartilage formation. Moreover, there was a large amount of immature chondrocytes and matrix with cartilage lacuna on PLGA/HAp/Zein scaffolds.
CONCLUSIONThe data suggest that the PLGA/HAp/Zein scaffolds possess good biocompatibility, which are anticipated to be potentially applied in cartilage tissue engineering and reconstruction.