A PCR-based Rapid Neutralization Assay for GII.4 Norovirus Infection in HIEC6 Cell Culture.

Yi Sun Fan; Cheng Liu; Hui Juan Zhu; Yi Ding; Wan Jie Zeng; Xu Fang Yin; Shuang Shuang Ding; Jun Zhang

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A PCR-based Rapid Neutralization Assay for GII.4 Norovirus Infection in HIEC6 Cell Culture.

Author: Yi Sun FAN ¹ ; Cheng LIU ² ; Hui Juan ZHU ³ ; Yi DING ⁴ ; Wan Jie ZENG ¹ ; Xu Fang YIN ¹ ; Shuang Shuang DING ¹ ; Jun ZHANG ⁵
Author Information

1. Clinical Laboratory, Suzhou Kowloon Hospital, Shanghai Jiao Tong University Medical School, Suzhou 215028, Jiangsu, China.
2. Suzhou Center for Disease Prevention and Control, Suzhou 215004, Jiangsu, China
3. Department of Epidemiology, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow University, Suzhou 215123, Jiangsu, China.
4. Suzhou Industrial Park Centers for Disease Control and Prevention (Institute of Health Inspection and Supervision), Suzhou 215021, Jiangsu, China.
5. Suzhou Center for Disease Prevention and Control, Suzhou 215004, Jiangsu, China.
Publication Type:Letter
MeSH: Antibodies, Neutralizing; immunology; Base Sequence; Caliciviridae Infections; diagnosis; virology; Case-Control Studies; Cell Line; DNA Primers; Enzyme-Linked Immunosorbent Assay; Gastroenteritis; diagnosis; virology; Humans; Norovirus; immunology; isolation & purification; Polymerase Chain Reaction; methods; Sensitivity and Specificity
From: Biomedical and Environmental Sciences 2015;28(3):219-221
CountryChina
Language:English
Abstract: Because of limited viral replication and lack of cytopathic effect in cell culture, a new PCR-based rapid seroneutralization assay for detection of GII.4 norovirus neutralized antibodies was developed with serum samples from acute-phase patients, convalescent-phase patients and healthy controls. According to this study, neutralizing antibodies were detected in 100% of convalescent-phase sera, and in 2.5% of healthy controls sera. However, all of the acute-phase serum samples could not neutralize virus efficiently. Compared to the results from ELISA (96.2% at sensitivity and 80% at specificity), the present in vitro neutralization assay is more specific and more sensitive.