Molecular Characteristics and Drug Susceptibility of Mycobacterium tuberculosis Isolates from Patients Co-infected with Human Immunodeficiency Virus in Beijing, China.

Jie Liu; Hui Zhu Wang; Lu Lu Lian; Yan Hua YU; Xiu Qin Zhao; Cai Ping Guo; Hai Can Liu; Shu Mei Liu; Hui Zhao; Zhao Ying Zeng; Xiu Ying Zhao; Kang Lin Wan

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Molecular Characteristics and Drug Susceptibility of Mycobacterium tuberculosis Isolates from Patients Co-infected with Human Immunodeficiency Virus in Beijing, China.

Author: Jie LIU ^{1
,

2
,

3} ; Hui Zhu WANG ⁴ ; Lu Lu LIAN ⁵ ; Yan Hua YU ⁶ ; Xiu Qin ZHAO ¹ ; Cai Ping GUO ⁶ ; Hai Can LIU ¹ ; Shu Mei LIU ⁶ ; Hui ZHAO ⁴ ; Zhao Ying ZENG ⁶ ; Xiu Ying ZHAO ⁷ ; Kang Lin WAN ¹ ;
Author Information

1. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China
2. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, China
3. Chaoyang Center for Disease Control and Prevention, Beijing 100021, China.
4. Beijing Ditan Hospital, affiliated to Capital Medical University, Beijing 100015, China.
5. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China.
6. Beijing You'an Hospital, affiliated to Capital Medical University, Bejing 100069, China.
7. Beijing You'an Hospital, affiliated to Capital Medical University, Bejing 100069, China
Publication Type:Letter
MeSH: AIDS-Related Opportunistic Infections; microbiology; Antitubercular Agents; pharmacology; China; Humans; Microbial Sensitivity Tests; Mycobacterium tuberculosis; classification; drug effects; isolation & purification; Phylogeny; Tuberculosis; complications; microbiology
From: Biomedical and Environmental Sciences 2015;28(3):222-226
CountryChina
Language:English
Abstract: 70 clinical Mycobacterium tuberculosis strains isolated from AIDS patients in two HIV/AIDS referral hospitals in Beijing were used in this study. M. tuberculosis and non-tuberculosis mycobacterium (NTM) were identified by using multi-locus PCR. M. tuberculosis was genotyped by using 15-locus MIRU-VNTR technique and spoligotyping afterwards. Meanwhile, the drug susceptibilities of the strains to the four first-line anti TB drugs (rifampin, isoniazid, streptomycin, and ethambutol) and the four second-line anti-TB drugs (capreomycin, kanamycin, ofloxacin, and ethionanide) were tested with proportional method. In this study, M. tuberculosis and NTM strains isolated from AIDS patients with TB-like symptoms were identified and genotyping analysis indicated that Beijing genotype was the predominant genotype. In addition, the prevalence of drug-resistant TB, especially the prevalence of XDR-TB, was higher than that in TB patients without HIV infection.