Visual detection of murray valley encephalitis virus by reverse transcription loop-mediated isothermal amplification.

Rui Gong; Han Hua Wang; Hong Qin; Xiao Ping Guo; Xue Jun MA

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Visual detection of murray valley encephalitis virus by reverse transcription loop-mediated isothermal amplification.

Author: Rui GONG ^{1
,

2} ; Han Hua WANG ³ ; Hong QIN ¹ ; Xiao Ping GUO ¹ ; Xue Jun MA ⁴
Author Information

1. Hubei International Travel Healthcare Center, Wuhan 430070, Hubei, China
2. National Key Lab of Ports Infectious Disease Detection, Hubei Entry-Exit Inspection and Quarantine Bureau of China, Wuhan 430050, Hubei, China.
3. Hubei International Travel Healthcare Center, Wuhan 430070, Hubei, China.
4. Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Publication Type:Letter
MeSH: Base Sequence; DNA Primers; Encephalitis Virus, Murray Valley; genetics; isolation & purification; Limit of Detection; Transcription, Genetic
From: Biomedical and Environmental Sciences 2015;28(3):227-230
CountryChina
Language:English
Abstract: A sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for rapid visual detection of Murray valley encephalitis virus (MVEV) infection. The reaction was performed in one step in a single tube at 63 °C for 60 min with the addition of the hydroxynaphthol blue (HNB) dye prior to amplification. The detection limit of the RT-LAMP assay was 100 copies per reaction based on 10-fold dilutions of in vitro transcribed RNA derived from a synthetic MVEV DNA template. No cross-reaction was observed with other encephalitis-associated viruses. The assay was further evaluated using spiked cerebrospinal fluid sample with pseudotype virus containing the NS5 gene of MVEV.